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Institute of Hydraulic Engineering - IWS

Fortgeschrittene Methoden in der Biofilm Forschung

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LectureFortgeschrittene Methoden in der Biofilm Forschung
(Advanced Methods in Biofilm Research)
LecturerLecture (V):Dr. rer.nat. Sabine Ulrike Gerbersdorf
Tutorial (Ü):Dr. rer.nat. Sabine Ulrike Gerbersdorf and Kaan Koca
Excursion (E):Dr. rer.nat. Sabine Ulrike Gerbersdorf
ContactDept. of Hydraulic Engineering and Water Resources Management
WorkloadSS: 1 V + Labor  (6 LP)Lecture No.021410206 + 021410206
VenueV 61.Sem. 1
Labor IWS
Time
Wed. 9:45 - 10:30 (Start: 18. October 2017)
Wed. 10:30 - 13:00 (Start: 18. October 2017)
Further information
  • M. Sc. Umweltschutztechnik, Studienrichtung Wasser, Masterfach Gewässerschutz & Wasserwirtschaft, Wahlmodul/Spezialisierungsmodul,3. oder 4. Semester
  • M.SC. WAREM, Vertiefungsrichtung S2 „Hydraulic Engineering and River Basin Mgt“, Wahlmodul, 3. Semester
  • Prerequisites
  • Basic experience in laboratory work as well as basic knowledge in ecology, microbiology, chemistry and physics
  • Basic knowledge in microscopy would be desirable
  • Description
  • Growth of a monocultural biofilm (bacterial origin) (cultures, nutrient broth, autoclaving)
  • Growth of a natural highly diverse biofilm (preparing substrates, circultating freshwater from river in mini-flumes)
  • Monitoring of biomass/cell numbers and growth (bacteria: Ultrasound, Staining, Flow Cytometry; Microalgae: Chlorophyll a Extraction, Photometer)
  • Extracellular polymeric substancen (EPS) : Extraction, Determination of sugar – and proteincontent in the matrix
  • Microscopy: Ligth-Microscopy to determine the most prominent species as well as address biofilm topography. Electron-Microscope to visualize various biofilm structures
  • Structure from Motion: Visualisation of three dimensional biofilm structures
  • Functionality of biofilm: Biostabilisation of sediments, measurements of the biofilm adhesion via MagPI (Magnetic Particle Induction)
  • Excursion to collect and observe biofilms in their natural habitat
  • Learning objectives
  • The students acquire fundamental knowledge of biofilms (composition, main species, requirements, occurrence, functionality and ecosystem services)
  • The students deepen their ability to work analytical in the laboratory on the micro- and macroscale
  • The students broaden their portfolio of applied methods ranging from autoclaving to photometry
  • The students apply microbiological techniques in freshwater ecology
  • The students learn about the most common biological and chemical parameters concerning biofilm research but also beyond
  • The students are familiar with light microscopy and get first hands-on experience with electron microscopy
  • The students know techniques to determine biofilm topography
  • He/she can measure biofilm adhesion with electromagnets
  • LiteratureBook: Biofilm Highlights (H.-C. Flemming, J. Wingender, SU. Szewzyk, Springer Verlag)
    More milestone publications (e.g. Reviews) will be recommended
    Classification -  021410205 Limnic Ecology
     -  021410103 Umweltgerechte Wasserwirtschaft
    ExamAcademic assessments: written, Laboratory protocols